Veterinary Microbiology

1. About The Department :

This department is operative since inception of the college in 1930 and 62 M.V.Sc. and Ph.D Scholar has been awarded degree in this discipline. More than 250 research publications in different national and international journals have been made. This department has undertaken 9 research projects funded by ICAR & other agencies out of which 2 are presently running. Resultantly the Department of Microbiology is very rich in infrastructure facilities. The staff in the Department of Veterinary Microbiology is engaged with teaching of UG and PG students of Veterinary Microbiology and applied research in diagnostic microbiology of animal diseases. They offer lectures, seminars and practical training to undergraduate and post graduate students wishing to pursue training in any combination of the following subjects:- Bacteriology, virology, mycology, immunology. The department has been active in the research on Foot and Mouth Disease, New Castle disease, Infectious Bursal Disease, Peste des petits Ruminat Infectious Bovine Rhinotracheitis, Mastitis, Mycotic mastitis and abortion, Blue tongue etc.The thrust area of research includes pathogenic bacteria, virus and fungi. The mission of this department is to promote development through high quality education, research, consultancy and extension services in the specialty fields of Microbiology, Molecular biology and Biotechnology. The beneficiaries include students of veterinary and animal science. The department offers scientific advice to all institutions that seek such specialized information. Outreach activities range from specialized diagnostic services to professional consultancy on the control of animal diseases of microbial origin.

2. Mandate / Objective :

  • To cater the needs of Undergraduate and Post graduate studies leading to B. V. Sc. &A.H., M. V. Sc. and Ph. D. in the discipline.
  • To carry out researches on different Bacteriological, Virological, Immunological and Mycological diseases in order to prevent and fight the existing dreadful and contagious diseases in order to prevent and fight the existing dreadful and contagious disease as  well as to find out measures to prevent and control the emerging disease caused by different micro organism.
  • Bacteriological, Virological and Immuniological and Mycological investigation of   materials received from different sources and thus aiding into diagnosis.
  • Educating livestock owners and farmers regarding various diseases of livestock and poultry through television, radio talk and popular article.

3. Faculty & Staff Position :

1. Dr. Manoj Kumar
Asstt. Professor
09835419031 drmanojmicro@rediffmail.com
2. Dr. Sudha Kumari
Asstt. Professor
09771265765  

3.

Dr. Savita Kumari

Asstt. Professor

09905874147

drsavitakumari@gmail.com

 

4. Teaching :

(i) U.G. Programme (as per VCI Syllabus)

Sl. No.

Course No.

Course Title

Cr.Hrs.

1

VMC-211

General Veterinary Microbiology

2+1

2

VMC-221

Veterinary Immunology and Serology

2+1

3

VMC-311

Veterinary Bacteriology  & Mycology

2+1

4

VMC-321

General  & Systemic Veterinary Virology

2+1

5

VLD-411

Laboratory diagnosis

0+2

6

VLD-511

Laboratory diagnosis

0+2

 

(ii) P.G.  Progrmme (as per ICAR Syllabus)

M.V.Sc. Courses :

Sl. No.

Course No.

Course Title

Credit hrs.

1

VMC 601

Bacteriology-I

3+1

2

VMC 602

Bacteriology-II

3+1

3

VMC 603

Veterinary Mycology

1+1

4

VMC 604

General Virology

2+1

5

VMC 605

Systematic Animal Virology

3+1

6

VMC 606

Principles of Immunology

2+1

7

VMC 607

Vaccinology

2+0

8

VMC 608

Diagnostics of infectious diseases

1+2

9

VMC 609

Techniques in microbiology and immunology

0+3

10

VMC 691

Master’s Seminar

1+0

 

 

Total

18+11=29

11

VMC 699

Master’s Research

20


Ph.D. Courses :

Sl. No.

Course No.

Course Title

Credit hrs.

1

VMC 701

Advances in Bacteriology

2+1

2

VMC 702

Advances in Mycology

2+1

3

VMC 703

Bacterial Genetics

2+1

4

VMC 704

Microbial Toxins

2+1

5

VMC 705

Molecular Determinants of Bacterial Pathogenesis

2+1

6

VMC 706

Advances in Virology

2+1

7

VMC 707

Molecular and Genetic aspects of Viral Pathogenesis

2+1

8

VMC 708

Structure function relationship of DNA and RNA virus

3+0

9

VMC 709

Oncogenic viruses

2+0

10

VMC 710

Slow viral infections and prions

2+0

11

VMC 711

Molecular immunology

2+1

12

VMC 712

Advances in cellular immunology

2+1

13

VMC 713

Cytokines and immunomodulators

2+0

14

VMC 714

Advances in vaccinology

2+0

15

VMC 715

Advances in immunodiagnostics

1+1

16

VMC 716

Modern immunotechnology

1+2

17

VMC 717

Current topics in infection and immunity

3+0

18

VMC 718

Veterinary microbial biotechnology

2+1

19

VMC 790

Special problems

0+2

20

VMC 791

Doctoral Seminar I

1+0

21

VMC 792

Doctoral Seminar II

1+0

Total :

37+15=52

22

VMC 799

Doctoral Research

45

N.B. : A Ph.D. student may also take 600 series courses of M.V.Sc. if not studied during Master’s programme.

5. Research :  

(i) Post Graduate Research (Curricular) :

M.V.Sc. Degree awarded :

Sl. No.

Name of the Student

Year

Title of the thesis

1.

Dr. S. C. Srivastava

1962

Antigenic structure of Pasteurella multocida type-I

2.

Dr. Sidheshwar Prasad

1963

Antigenic structure of Bacillus anthracis

3.

Dr. N. C. Nag

1964

Serological and Physiological characters of staphylococci isolated from bovine udder.

4.

Dr. S. A. Ansari

1965

Study of the prevalence of E. Coli and Salmonella in the intestinal tract of calves in health and in enteric disorders.

5.

Dr. A. Kumar

1965

Aerobic bacterial flora of upper respiratory tract of calves in health and in enteric disorders.

6.

Dr. G. Saran

1965

Bacteriological studies on suspected cases of infertility in cattle and sheep with particular reference to Vibrio, Brucella and PPLO.

7.

Dr. M. N. Sinha

1966

Study of intestinal clostridia of goats with special reference to Clostridium welchii.

8.

Dr. A. N. Narulla

1966

Study of Aerobic bacterial flora of gastro intestinal tract of poultry in health and disease with special reference to E.coli

9.

Dr. K. P. Reddy

1966

Studies on bacterial flora of respiratory tract of poultry with special reference to PPLO

10.

Dr. C. R. Prasad

1967

Study of the staphylococcal flora in milk from apparently healthy udder.

11.

Dr. S. G. Mullick

1967

Study of the aerobic flora of public health importance in goats.

12.

Dr. B. K. Tiwary

1967

Entero pathogens of cattle, goats, and poultry with a view to determine the epidemiology.

13.

Dr. M. A. Mangyaulli

1967

Bacterial flora of respiratory tract of cattle and goats.

14.

Dr. A. K. Sinha

1970

An experimental study of combined vaccine against H.S. and B.Q.

15.

Dr. H. N. Verma

1971

Experimental studies on the pathology of fowl pox in chickens.

16.

Dr. Sabir Anjum

1971

Bacterial flora including Mycobacteria of the respiratory tract of cattle and buffalo in Bihar.

17.

Dr. A. P. Verma

1971

Studies on the incidence of Salmonella and Escherichia coli in sheep and goats in health and enteric disorders.

18.

Dr. P. K. Mohanty

1973

Studies on E. coli and Salmonella infections in poultry.

19.

Dr. S. N. Sinha

1973

Detailed studies of certain serotypes of E. coli especially associated in colibacillosis in poultry.

20.

Dr. Mohammed Sayeed

1973

Bacteriological study of bovine mastitis.

21.

Dr. K. P. Dhakal

1974

Detailed studies of certain serotypes of E. coli especially associated with calf.

22.

Dr. P. Dutta

1975

Immunological response in blood sera of buffalo calves and rabbits against Pasteurella multocida using different adjuvants.

23.

Dr. P. C. Verma

1975

Microbiological study of eternal ear of dogs and buffaloes in healthy and in diseases condition (Otitis externa) in Bihar with special reference to antibiotic sensitivity test in vitro.

24.

Dr. Ashok Kumar

1977

Studies on enteric infection in calves with special reference to Salmonellosis.

25.

Dr. V. K. Mehrotra

1977

A comparative study of bacterial flora of the genital tract of healthy and infertile cows, buffaloes and antibiotic sensitivity tests of the isolates.

26.

Dr. N. K. Sharma

1978

Bacteriological examination of the dead shell chick embryos which die during incubation in and around Patna.

27.

Dr. R. C. Sinha

1978

Correlation of age, sex and breed of cattle and buffaloes of organizes farms and goshalas of Bihar with respect of tuberculin reaction.

28.

Dr. V. Choudhary

1979

Bacteriological quality of bull semen.

29.

Dr. V. K. Sinha

1979

Bacteriological studies on bovine and canine conjunctivae in healthy and diseased condition.

30.

Dr. Dharmendra Sinha

1980

Carrier state of Salmonella and Citrobacter infection in calves, goats and its public health importance.

31.

Dr. J. C. Debnath

1980

Effect of Pasteurella multocida and its endotoxins on blood coagulation and electrolyte in buffalo calves and rabbits.

32.

Dr. Arun Prasad

1980

Studies on the aerobic bacteria and fungi of respiratory tract of poultry in health and disease.

33.

Dr. Shyama Prasad

1981

Study on the association of fungi with female genital tract of cattle in health and genital disorders.

34.

Dr. A. K. Sinha

1984

Studies on mycoflora of respiratory tract of goats in healthy and disease.

35.

Dr. Bipin Kumar

1984

Studies on Mycoplasma from the genital and respiratory tracts of cattle and buffaloes.

36.

Dr. Pramod Kumar

1984

Studies on comparative efficacy of different techniques for the detection of foot and mouth disease virus.

37.

Dr. S. Brahamachary

1985

Studies on the antibody response of cattle to FMD virus type ‘Asia-I’ after infection and vaccination.

38.

Dr. U. K. Gupta

1985

Studies on the immune response of cattle to FMD virus types ‘O’, ‘A’, ‘C’, and ‘Asia-I’ after vaccination.

39.

Dr. Sanjay Kumar Sinha

1987

Study on cell mediated immune response in mice to FMD virus serotype “O”.

40.

Dr. (Mrs.) Neelam Kumari

1987

Study on fungi associated with poultry feeds with particular reference to Aflatoxin and its in-vivo effect.

41.

Dr. Adarsha Pradhan

1991

Isolation and characterization of Mycoplasmas from the respiratory tracts of goats in health and disease.

42.

Dr. Sunil Kumar Verma

1991

Studies on infectious Bursal Disease virus infection in chickens with reference to seroprevalence, Immunosuppression and Immunomodulation.

43.

Dr. Jitendra Kumar Singh

1993

Seroprevalence and pathogenicity of Egg drop syndrome 1976 virus in chickens.

44.

Dr. Kumud Kumar

1997

Prevalence of maternal antibody and its influence on the vaccination against infectious bursal disease in chickens.

45.

Dr. Sunil Kumar

1997

Seroprevalence of Ranikhet disease in unvaccinated poultry and pathogenicity of avirulent R.D. virus in immunosuppressed chickens.

46.

Dr. Anjali Kumari Sinha

1997

Seroprevalence, Isolation & Immunosuppressive effect of infectious Bursal disease virus in chickens.

47.

Dr. Nitesh Kumar

1998

Effect on Immune system of experimentally induced Aflatoxicosis is rabbits.

48.

Dr. Sanjay Kumar

1998

Immune response of Invasive intermediate strain infectious bursal disease virus vaccine in broiler chickens.

49.

Dr. B. B. Khadka

1999

Studies on Aflatoxicosis of buffalo calves with special reference to Humoral and cell mediated immune response.

50.

Dr. Girish Kumar Sinha

2000

Studies on Aflatoxin contamination in poultry feeds and role of some indigenous substances on Aflatoxin productions.

51.

Dr. Mithilesh Kumar

2000

Effect of selected agents on immunomodulation in chickens immunocompromised by a vaccine strain of infections bursal disease virus.

52.

Dr. Subhash Chandra Hindustani

2000

Efficacy of certain agents on enhancement of immune responses to Intermediate plus infections Bursal disease virus vaccine in Broiler chickens.

53.

Dr. Sanjeev Kumar Tiway

2000

Studies on level of Aflatoxin in cattle in relation to certain clinical conditions.

54.

Dr. (Mrs.) Rekha Teresa Kujur

2001

Evaluation of certain Immunomodulatory agents in countering immunosuppressive effects of a vaccine strain of infectious Bursal disease virus in chicken.

55.

Dr. (Mrs.) Anamika

2002

Effects of Aflatoxin on immune response to invasive intermediate strain IBD viral vaccine in broiler chicken.

56.

Dr. Manoj Kumar

2002

Efficacy of certain agents in controlling immunosuppressive effects of Infectious Bursal Disease Virus in Chickens.

57.

Dr. Alok Singh

2003

Studies on Seroprevalence of Infectious Bovine Rhinotracheitis in Bovine in and around Patna.

58.

Dr. Bhagirath Prasad

2003

Distribution of antibody titre to Ranikhet disease virus in Immunocompromised chicken.

59.

Dr. Jay Prakesh

2003

Studies on seroprevalence of Bovine Brucellosis in and around Patna.

60.

Dr. Umesh Prasad

2004

Serotype and Antimicrobial susceptibility of E. coli from digestive tract of pigs.

61.

Dr. Shashi Sinha

2004

Studies on prevalence of FMD virus serotypes in and around Patna.

62.

Dr. Akhileshwar Thakur

2004

Comparative serological studies on caprine brucellosis in and around Patna.

63.

Dr. Shashi Bhushan Sudhakar

2006

Isolation and identification of dermatophytes in apparently healthy skin and skin with clinical lesion in different breeds of dogs.

64.

Dr. R. S. Rajmani

2006

Studies on the association of microbes in eggs with special reference to toxigenic fungi.


Ph.D. Degree awarded :

Sl. No.

Name of the Student

Year

Title of the thesis

1.

Dr. Manoj Kumar

2005

Antigenic, pathogenic, immunogenic and molecular characterization of very virulent infectious bursal disease virus.


(ii) Research Projects:

List of research project undertaken :

Sl. No.

Name of the Research Project

Funding Agency

1

Studies on aflatoxin in milk and milk products in different agro-climatic areas of Bihar

ICAR, New Delhi

2

Animal health information System through Disease Monitoring and Surveillance

ICAR, New Delhi

3

Studies on Immunobiology and pathology of aflatoxin in animals and men in Bihar including its tribal areas

ICAR, New Delhi

4

Studies on epidemiology, treatment and prevention of gangrenous syndrome in rural buffaloes and cattle in Bihar

ICAR, New Delhi

5

Comparative evaluation of different agents in controlling immunosuppressive effects of infectious Bursal Disease virus in chickens

ICAR, New Delhi

6

To study the unresponsiveness of Kalaazar patients to antileishmania

D.S.T., New Delhi

7

International mycotoxin cheque sample programme

W.H.O., Geneva

8

Systematic study of bacterial isolates from milk samples, effect of microbial contamination of the milk and their Public Health importance (JARO)

ICAR, New Delhi

9

All India co-ordinated Research Project for Epidemiological Studies on Foot and Mouth disease.

ICAR, New Delhi

10

Studies on mycotoxin from livestock and the effect of aflatoxin on immune response of poultry and laboratory animals.

RAU, Pusa

11

Immune profile of buffalo in health and disease

RAU, Pusa

12

Investigation into mycotic disease of livestock and zoo animals

RAU, Pusa

13

Studies on the enzymatic activity of fungi

RAU, Pusa

14

Association of fungi in the genital tract of infertile cows and buffaloes

RAU, Pusa

15

Survey and control of tuberculosis in livestock and poultry. Execution of its control and eradication programme (JARO)

Govt. of Bihar, Patna, Bihar


Ongoing research Projects (Externally Funded) :

Sl. No.

Name of the Research Project

Funding Agency

1

All India Coordinated Research Projects for Epidemiological Studies on Foot and Mouth Disease

ICAR, New Delhi

2

Studies on reproductive diseases of cattle under field condition of Bihar”

ICAR, New Delhi


(iii) Research Achievements
:

  • Altogether 1853 samples comprising 594 (37.88%) human food, 368 (51.63%) animal feed, 78 (56.4%) poultry feeds, 82 (13.42%) meat samples, 328 (18.90%) animal biological fluid and 403 (17.16%) human biological fluids were collected and processed for aflatoxin assay. The aflatoxin were estimated by feeding the toxin in buffalo and haematological biochemical and immunological profile were studied. Histopathological studies of different organ were studied with special emphasis on liver. The result was indicative of typical impact of aflatoxicosis.
  • For the active surveillance sera samples were collected from 431 animals. Out of which 3679 were cattle, 1810 were buffalo and 256 goats. Till to-day 360 sera samples were subjected to AB-ELISA test to detect antibodies against Brucalla and Infectious Bovine Rhinotracheitis (IBR).
  • The network unit of AICRP on FMD is operative from November, 2000 and till date 934 FMD outbreaks have been studied covering cattle, buffalo, goat, sheep and pigs, out of which 771 were found positive and serotyped. The prevalence rate of FMD employing 3AB3 NSP based ELISA (DIVA) has been done and the prevalence rate of FMD was established to the tune of 26.71%.

6. Extension :

  • Advisory to the farmers pertaining to prevention and control of important infectious disease including foot and mouth disease.
  • Laboratory diagnosis of bacterial, viral and fungal diseases and culture sensitivity of suspected samples.

 7. Publications:  

Research : National - 250, International – 15, Books/Manuals : 06

8. Facilities :

  • Detection of aflatoxin in feed and biological fluids.
  • Immunological techniques like, HA, HI, Sandwich ELISA, LPB ELISA and Recombinant 3AB3 NSP based ELISA (DIVA test).
  • Isolation and identification of aerobic and anaerobic bacteria.

9. Seminar/Symposia organized :

National symposium on Aflatoxicosis in relation to human and animal health was held on 11th & 12th June, 1989, Bihar Veterinary College, Patna-14

10. Any other information :

  • Assisted the scientist of Livestock and Fisheries Improvement and Management Programme (LFIMP),ICAR Complex for Eastern Region, B.V.College, Patna)  in the project “Studies on reproductive diseases of cattle under field condition of Bihar”.
  • The pre and post vaccination serum samples were collected as per the protocol. The antibody titre assessment was done employing LPB ELISA.
  • The screening of milk of cow suffering from mastitis was carried out which included isolation and identification of causative organism and antibiotic sensitivity test.
  • Trial of FMD monovalent vaccine (′O′ strain) has been approved by the Project Directorate on Foot and Mouth Disease (PD-FMD), IVRI Campus, Mukteshwar. It will commence its strain selection; formulation and farm animal model trial as part of the National FMD control programme as per the OIE/FAO vision.